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Prediction of Transmembrane Regions and Orientation


The TMpred program makes a prediction of membrane-spanning regions and their orientation. The algorithm is based on the statistical analysis of TMbase, a database of naturally occurring transmembrane proteins. The prediction is made using a combination of several weight-matrices for scoring.
  • K. Hofmann & W. Stoffel (1993)
    TMbase - A database of membrane spanning proteins segments
    Biol. Chem. Hoppe-Seyler 374,166

For further information see the TMbase documentation.



  • Usage: Paste your sequence in one of the supported formats into the sequence field below
    and press the "Run TMpred" button.
    Make sure that the format button (next to the sequence field) shows the correct format
    Choose the minimal and maximal length of the hydrophic part of the transmembrane helix

Output format

minimum maximum

Query title (optional)

Input sequence format

Query sequence:
or ID or AC or GI
(see above for valid formats)